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    Equipe : Biology of Phagocytes

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     Responsable


    CV Aviesan

    Phagocytosis is the mechanism of capture and degradation of pathogens or debris that is performed by professional cells of the immune system. In certain circumstances, this process is impaired, which can lead to the development of opportunistic pathogens or chronic inflammation. We study the mechanims of capture and degradation of phagocytic cells, their impact on immune responses and their alterations by viral infections such as the Human Immunodeficiency Virus (HIV-1).

     

    Objectives


    Professional phagocytes are specialized cells of the immune system that play a major role in innate and adaptive immune responses. Phagocytosis and degradation of invading microorganisms or debris is crucial for bacterial clearance and resolution of inflammation. In addition, presentation of antigens and cytokine secretion, which are late events determined by the pathway of internalization, are key elements of the immune responses. Therefore, it is crucial to understand the mechanisms of phagocytosis, especially of cell debris or microorganisms.

    Our goal is to dissect the mechanisms used by phagocytes and in particular the coordinated activities of signaling pathways, membrane trafficking and cytoskeleton dynamics and their effect on the outcome of immune responses. We also analyze precisely the mechanisms that allow pathogen development in HIV-infected macrophages. Finally, we recently showed that part of the internalized material can be regurgitated without degradation and captured by B lymphocytes, a process that we aim to characterize further to better understand and manipulate the humoral immune response.

     

    Main publications :

     

    Marie-Anaïs F, Mazzolini J, Herit F and Niedergang F. Dynamin-actin cross-talk contributes to phagosome formation and closure. Traffic (2016) 17 : 487-499.

    Niedergang F, Di Bartolo V, Alcover A. Comparative Anatomy of Phagocytic and Immunological Synapses. Front Immunol. (2016) Jan 28;7:18. Review.

    Niedergang F., Phagocytosis. In: Ralph A Bradshaw and Philip D Stahl (Editors-in-Chief), Encyclopedia of Cell Biology, Vol 2, Waltham, MA: Academic Press, 2016, pp. 751-757.

    Dumas A, Lê‑Bury G, Marie‑Anaïs F, Herit F, Mazzolini J, Guilbert T, Bourdoncle P., Russell DG, Benichou S,  Zahraoui A,  and Niedergang F. The HIV-1 protein Vpr impairs phagosome maturation by controlling microtubule-dependent trafficking.
    J. Cell Biol. (2015) 211 : 359-372

    Deschamps C, Echard E and Niedergang F. Phagocytosis and cytokinesis: do cells use common tools to cut and to eat ? HIghlights on common themes and differences. Traffic (2013) 4: 355-364. Review.

    Marion S., Mazzolini J., Herit F., Bourdoncle P., Kambou-Pene N., Hailfinger S., Sachse M., Benmerah A., Echard A., Thome M. and Niedergang F. The NF-κB signaling protein Bcl10 regulates actin dynamics by controlling AP1 and OCRL-bearing vesicles. Dev Cell (2012) 23 : 954-967.

     

    Team's news

     

    • The team obtained an ANR grant PHAGOMECANO to work on « Mechanotransduction associated with integrin-mediated phagocytosis »
    • FN will co-organize the 20th annual meeting of the Club Exocytose-Endocytose, to be held in Giens in May 2017
    • Our 2016 Traffic paper on phagosome closure was highlighted on the CNRS website
    • Thierry Lhermitte who supports the Fondation pour la Recherche Medicale, visited the lab in March 2016. The interview came out in Paris Match.
    • Our 2015 JCB paper was highlighted on the CNRS websites and the regional and national Inserm sites
    • The lab participated to the “Fête de la Science” event on October 9th for class visit and October 10th (activity “How sentinel cells become a shelter for microbes?”.
    • The team obtained the « Equipe FRM » (Team FRM) label from Fondation pour la Recherche Médicale (2013-2017).