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    Team: Cell signaling in bacterial infections.


    Team leader


    Bacterial infections represent a rising threat on human health. They result from complex molecular interactions between a large number of microbial components and host factors, and therefore, need to be addressed on a global scale. Using the enteroinvasive bacterium Shigella flexneri as main model of infection, we combine systems biology methods and mechanistic approaches to characterize the host molecular pathways involved in several key aspects of bacterial infections including invasion of epithelial cells, host cell survival and inflammation.



    The emergence of multi-drug-resistance mechanisms among major pathogenic bacteria represents a rising threat on human health. The lack of new therapeutic options requires an urgent effort of the scientific community to identify new microbial targets and envision alternative strategies. A promising approach consists of targeting the host instead of the pathogen. Indeed, it became clear over the last years, that pathogens subvert key cellular factors and responses in order to infect their host. Blocking the expressing of these factors or interfering with their activities may prevent colonization and have a positive impact on the infection outcome. Since infections result from countless molecular interactions between microbial components and host factors, identifying key cellular targets requires mapping these interactions on a global scale.

    Using the enteroinvasive bacterium Shigella flexneri as main model, we apply systems biology methods including large scale RNAi screens and phosphoproteomics to identify and characterize the cellular pathways that are required for bacterial invasion of epithelial cells, survival of infected cells and inflammation, three central aspects of S. flexneri pathogenesis.



    Main publications

    Milivojevic M, Dangeard AS, Kasper CA, Tschon T, Emmenlauer M, Pique C, Schnupf P, Guignot J, Arrieumerlou C. ALPK1 controls TIFA/TRAF6-dependent innate immunity against heptose-1,7-bisphosphate of gram-negative bacteria. PLoS Pathog (2017) Feb 21;13(2):e1006224.

    Gaudet RG, Guo CX, Molinaro R, Kottwitz H, Rohde JR, Dangeard AS, Arrieumerlou C, Girardin SE, Gray-Owen SD. Innate Recognition of Intracellular Bacterial Growth Is Driven by the TIFA-Dependent Cytosolic Surveillance Pathway. Cell Rep (2017), May 16;19(7):1418-1430.

    Ittig SJ, Schmutz C, Kasper CA, Amstutz M, Schmidt A, Sauteur L, Vigano MA, Low SH, Affolter M, Cornelis GR, Nigg EA, Arrieumerlou C. A bacterial type III secretion-based protein delivery tool for broad applications in cell biology. J Cell Biol. (2015) Nov 23;211(4):913-31.

    Schmutz C, Ahrné E, Kasper CA, Tschon T, Sorg I, Dreier RF, Schmidt A, Arrieumerlou C. Systems-level overview of host protein phosphorylation during Shigella flexneri infection revealed by phosphoproteomics. Mol Cell Proteomics. 2013 Oct; 12(10):2952-68.

    Kasper, C. A., Sorg, I., Schmutz, C., Tschon, T., Wischnewski, H., Kim, M. L. and Arrieumerlou, C. (2010). Cell-Cell Propagation of NF-kappaB Transcription Factor and MAP Kinase Activation Amplifies Innate Immunity against Bacterial Infection. Immunity, 33(5), 804–816.


    Team's news

    • The lab obtained funding from ARC to work on detection mechanisms of Helicobacter pylori infection in stomach epithelial cells (2018-2019).
    • Milica Milivojevic, first PhD student of the lab in Paris, successfully defended her PhD! Very well done, congratulations!
    • The HBPsensing project, coordinated by CA, was positively evaluated by the ANR (2018-2020). Thanks to L. Mulard, J. Enninga and I. Gomperts-Boneca to be part of this collaborative project.
    • We welcome Diego Garcia Weber to our lab. Diego will work as a postdoctoral fellow.
    • Milica Milivojevic obtained the APEM price awarding the best articles by young scientists of the Cochin Institute.
    • Charlène Dauriat successfully obtained her Master diploma. Congratulations!
    • Cécile Arrieumerlou obtained the “Accueil de chercheurs de haut niveau” grant from ANR (2015-2019).