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    Team: Biology of Phagocytes

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    Team leader
     

    CV Aviesan

    Phagocytosis is the mechanism of capture and degradation of pathogens or debris that is performed by professional cells of the immune system. In certain circumstances, this process is impaired, which can lead to the development of opportunistic pathogens or chronic inflammation. We study the mechanims of capture and degradation by phagocytic cells, their impact on immune responses and their alterations by viral infections, leading to the development of bacterial co-infections.

     

    Objectives

    Professional phagocytes play a major role in innate and adaptive immune responses. Phagocytosis and degradation of invading microorganisms or debris is crucial for bacterial clearance and resolution of inflammation. Therefore, it is crucial to understand the mechanisms of phagocytosis, especially of cell debris or microorganisms.

    Our first goal is to dissect the mechanisms used by phagocytes, in particular the coordinated activities of signaling pathways, membrane trafficking and cytoskeleton dynamics, and their effect on the outcome of immune responses. Second, we analyze how HIV infection or respiratory viruses associated with lung inflammation impair the phagocytic functions. Finally, we will explore further how dendritic cells regurgitate non-degraded material to activate B cells in order to manipulate the humoral immune response.


    Main publications :

    Mularski A, Marie-Anaïs F, Mazzolini J, Niedergang F. Observing Frustrated Phagocytosis and Phagosome Formation and Closure Using Total Internal Reflection Fluorescence Microscopy (TIRFM). Methods Mol Biol. (2018);1784:165-175

    Lê-Bury G and Niedergang F Defective phagocytic properties of HIV-infected macrophages: how might they be implicated in the development of invasive Salmonella Typhimurium ? Front. Immunol. (2018) 9:531

    Niedergang, F. and Grinstein, S. How to build a phagosome: new concepts for an old process. Curr Op Cell Biol  (2018) 50:57-63

    Jubrail, J., Kurian, N. and Niedergang, F. Macrophage phagocytosis cracking the defect code in COPD. Biomedical J. (2017) 40 : 305-312

    Marie-Anaïs F, Mazzolini J, Herit F and Niedergang F. Dynamin-actin cross-talk contributes to phagosome formation and closure. Traffic (2016) 17 : 487-499.

    Niedergang F., Phagocytosis. In: Ralph A Bradshaw and Philip D Stahl (Editors-in-Chief), Encyclopedia of Cell Biology, Vol 2, Waltham, MA: Academic Press, 2016, pp. 751-757.

    Dumas A, Lê‑Bury G, Marie‑Anaïs F, Herit F, Mazzolini J, Guilbert T, Bourdoncle P., Russell DG, Benichou S,  Zahraoui A,  and Niedergang F. The HIV-1 protein Vpr impairs phagosome maturation by controlling microtubule-dependent trafficking.
    J. Cell Biol. (2015) 211 : 359-372

    Marion S., Mazzolini J., Herit F., Bourdoncle P., Kambou-Pene N., Hailfinger S., Sachse M., Benmerah A., Echard A., Thome M. and Niedergang F. The NF-κB signaling protein Bcl10 regulates actin dynamics by controlling AP1 and OCRL-bearing vesicles. Dev Cell (2012) 23 : 954-967.

     

    Team's news

    • We propose several projects for masters / PhD students. Don’t hesitate to contact the lab !

           Some projects available here : https://cri-paris.org/aiv-internship/integrated-mechanobiology-of-integrin-mediated-phagocytosis-2/
          Or fiche_imvi 18-19
           Physics M2_2018-2019

    • Jamil Jubrail was awarded 1st prize for his presentation in a competition with other post-docs at the British Association of Lung Research Summer Meeting 2018, Birmingham, UK in September: congratulations!
    • Anna Mularski obtained a travel grant to attend the ASCB / EMBO meeting next December in San Diego : congratulations !
    • Our manuscript on inhibition of CCR5 secretion and HIV infection is available on BioRXiv.You can access it and share it via https://www.biorxiv.org/content/early/2018/07/08/364927
    • Florence Niedergang co-organizes the 3rd International Building the Cell meeting in Paris, September 26-28, 2018 http://www.atoutcom.com/btc2018/
    •  The lab has participated to the « open doors » of the « Hôpital Cochin » on May 26, 2018 and will participate to the “Fête de la Science” event on October 12th for class visits and on October 13th for general public (activity “How sentinel immune cells become a shelter for microbes?”)
    • Florence Niedergang and Jamil Jubrail organized the 8th Institut Cochin Miltenyi symposium on « Respiratory diseases » on November17, 2017 https://institutcochin.fr/animation/symposium