Biomedical research institute
     

    Methodological approaches

    •  

     

    - In vivo  analysis :

     

     

     - Various types of neuronal cultures in vitro :

    • ex vivo using organotypic cultures of hinbrains or slices of CNS;
    • collagen assays using explants from the  CNS faced with cells or tissues secreting diffusible factors to be challenged for their guidance properties
    • cell cultures of dissociated neurons.

     

    - Pharmacological approaches: treatment with bacterial toxins or chemicak coumpounds to deciper signaling pathways.

    - Life imaging of axon and cell bodies movements.

    - Transfection using electroporation of expression plasmids in post-mitotic neurons in nervous system or in dissociated neurons..