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    Neuro-anatomic analysis using in toto immunofluorescence on diaphragm

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    Immunofluorescence with an anti-peripherin (intermediate filament) antibody that specifically labels motor axons. Illustration on E18.5 diaphragm stained in toto, wild-type or mutant for CLIP3/CLIPR-59 expression Couesnon et al., 2013).