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    Role of intracellular compartments and of microtubules for axon guidance, outgrowth and stabilization.

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    We have initiated projects that aim at characterizing the role of microtubules through the analysis of mutant mice with a defective microtubules dynamics. We are interested in characterizing the consequences of stabilization defects in the microtubule cytoskeleton on the cross-talk between microtubules and actin in response to guidance factors. We also study the role of intracellular organelles during neuronal migration.


    We have shown that tubulin tyrosination by the tubulin tyrosin ligase enzyme is required for the proper organization of the growth cone and its pathfinding during embryonic development (Marcos et al., 2009b).

    We have also studied the role of a protein that belongs to the CLIP family (proteins that are able to bind microtubules), calles CLIP3/CLIPR-59, in a mouse KO model that we have generated. CLIP3 is particularly expressed in teh CNS, in particular in precerebellar neurons and motoneurons; its expression increases during embryonic development. CLIP3 is required for the stabilization of neuro-muscular junctions at late embryonic stages and perinatally (Couesnon et al., 2013).

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    Dissociated precerebellar neurons have been cultured for 2 days.

    Both actin (in red) and microtubules (in green) have been revealed by immunofluorescence

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