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    The mechanism of phagosome closure unravelled

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    The team of Florence Niedergang reveals the mechanism implicated in the closure of phagosomes

    The team of Florence Niedergang reveals the mechanism implicated in the closure of phagosomes, the compartments formed after the ingestion of microbes or cell debris by macrophages.

    Phagocytosis was described by Elie Metchnikoff, and 2016 marks the centenary of his death. Despite this description the mechanism of phagosome closure has remained unknown.

    The team set up an original experimental approach that allows visualisation with high precision the exact steps of phagosome formation and closure in living macrophages. The method is based on the Total Internal Reflection Fluorescence (TIRF) Microscope available in the Photonic Cellular Imaging facility at the Institut Cochin.

    The researchers demonstrate that dynamin-2 plays a crucial role in phagosome formation and closure. They highlight a cross-talk between dynamin activity and actin polymerization. This cooperation, which relies on a molecular interaction between dynamin-2 and actin, is required for efficient phagocytosis. The results are published in Traffic.

    Niedergang F_phagosome closure

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    Dynamin-actin cross-talk contributes to phagosome formation and closure.
    Florence Marie-Anaïs, Julie Mazzolini, Floriane Herit and Florence Niedergang
    Traffic (2016) Feb 5. Doi :10.1111/tra.12386

    Researcher contact

    Florence Niedergang
    Institut Cochin

    CNRS UMR8104, Inserm U1016, Université Paris Descartes
    
22, rue Méchain, 75014 Paris - France
    33(0)1 40 51 64 21
    florence.niedergang@inserm.fr