Institut de recherche biomédicale

    Publications 2001-2018


    Pagesy, Patrick; Tachet, Caroline; Mostefa-Kara, Ali; Larger, Etienne and Issad, Tarik.
    Increased OGA Expression and Activity in Leukocytes from Patients with Diabetes: Correlation with Inflammation Markers.
    Exp Clin Endocrinol Diabetes. 2018 Jun 11.

    Fougerat A, Pan X, Smutova V, Heveker N, Cairo CW, Issad T, Larrivée B, Medin JA, Pshezhetsky AV
    Neuraminidase 1 activates insulin receptor and reverses insulin resistance in obese mice.
    Mol Metab. 2018 Jun;12:76-88.

    Ramos-Martinez I, Martínez-Loustalot P, Lozano L, Issad T, Limón D, Díaz A, Perez-Torres A, Guevara J, Zenteno E.
    Neuroinflammation induced by amyloid β25-35 modifies mucin-type O-glycosylation in the rat's hippocampus.
    Neuropeptides. 2018 Feb;67:56-62


    Gondoin A, Hampe C, Eudes R, Fayolle C, Pierre-Eugène C, Miteva M, Villoutreix BO, Charnay-Pouget F, Aitken DJ, Issad T, Burnol AF.
    Identification of insulin-sensitizing molecules acting by disrupting the interaction between the Insulin Receptor and Grb14.
    Sci Rep. 2017 Dec 4;7(1):16901

    Groussaud D, Khair M, Tollenaere AI, Waast L, Kuo MS, Mangeney M, Martella C, Fardini Y, Coste S, Souidi M, Benit L, Pique C, Issad T.
    Hijacking of the O-GlcNAcZYME complex by the HTLV-1 Tax oncoprotein facilitates viral transcription.
    PLoS Pathog. 2017 Jul 24;13(7):e1006518.

    Marciniak, E., A. Leboucher, E. Caron, T. Ahmed, A. Tailleux, J. Dumont, T. Issad, E. Gerhardt, P. Pagesy, M. Vileno, C. Bournonville, M. Hamdane, K. Bantubungi, S. Lancel, D. Demeyer, S. Eddarkaoui, E. Vallez, D. Vieau, S. Humez, E. Faivre, B. Grenier-Boley, T. F. Outeiro, B. Staels, P. Amouyel, D. Balschun, L. Buee, and D. Blum.
    Tau deletion promotes brain insulin resistance.
    J Exp Med. 2017 214(8):2257-2269

    Voir aussi : INSERM actualités


    Pagesy P, Fardini Y, Nguyen TT, Lohmann M, Pierre-Eugene C, Tennagels N, Issad T.
    Effect of insulin analogues on phosphatidyl inositol-3 kinase/Akt signalling in INS-1 rat pancreatic derived β-cells.
    Arch Physiol Biochem. 2016;122(2):54-60.


    Fardini Y, Perez-Cervera Y, Camoin L, Pagesy P, Lefebvre T, Issad T.
    Regulatory O-GlcNAcylation sites on FoxO1 are yet to be identified.
    Biochem Biophys Res Commun. 2015 Jun 462(2):151-8

    Baudoin L, Issad T
    O-GlcNAcylation and Inflammation: A Vast Territory to Explore.
    Front Endocrinol (Lausanne). 2015 5:235.

    Lefebvre T, Issad T
    30 Years Old: O-GlcNAc Reaches the Age of Reason - Regulation of Cell Signaling and Metabolism by O-GlcNAcylation.

    Front Endocrinol (Lausanne). 2015 6:17.


    Kuo, M-S. , Auriau, J. , Pierre-Eugene, C., Issad, T.
    Development of a Human Breast-Cancer Derived Cell Line Stably Expressing a Bioluminescence Resonance Energy Transfer (BRET)-Based Phosphatidyl Inositol-3 Phosphate (PIP3) Biosensor.
    PLoS ONE 2014; 9(3):e92737

    Fardini Y, Masson E, Boudah O, Ben Jouira R, Cosson C, Pierre-Eugene C, Kuo MS, Issad T.
    O-GlcNAcylation of FoxO1 in pancreatic β cells promotes Akt inhibition through an IGFBP1-mediated autocrine mechanism.
    FASEB J. 2014 Feb;28(2):1010-21


    Dridi L, Seyrantepe V, Fougerat A, Pan X, Bonneil E, Thibault P, Moreau A, Mitchell GA, Heveker N, Cairo CW, Issad T, Hinek A, Pshezhetsky AV.
    Positive regulation of insulin signaling by neuraminidase 1.
    Diabetes. 2013 Jul;62(7):2338-46.

    Fardini Y, Dehennaut V, Lefebvre T, Issad T.
    O-GlcNAcylation: A New Cancer Hallmark?
    Front Endocrinol (Lausanne). 2013 Aug 12;4:99.

    Kanwal S, Fardini Y, Pagesy P, N'tumba-Byn T, Pierre-Eugène C, Masson E, Hampe C, Issad T.
    O-GlcNAcylation-inducing treatments inhibit estrogen receptor α expression and confer resistance to 4-OH-tamoxifen in human breast cancer-derived MCF-7 cells.
    PLoS One. 2013 Jul 11;8(7):e69150.


    Kazantzis M, Takahashi V, Hinkle J, Kota S, Zilberfarb V, Issad T, Abdelkarim M, Chouchane L, Strosberg AD.
    PAZ6 cells constitute a representative model for human brown pre-adipocytes.
    (2012) Front Endocrinol (Lausanne), 3:13.

    Pierre-Eugene, C., Pagesy, P., Nguyen, T.T., Neuillé, M., Tschank, G., Tennagels, N., Hampe, C., Issad, T.
    Effect of insulin analogues on insulin/IGF1 hybrid receptors: Increased activation by glargine but not by its metabolites M1 and M2
    (2012) PLoS ONE, 7 (7), art. no. e41992.

    Zheng, H., Worrall, C., Shen, H., Issad, T., Seregard, S., Girnita, A., Girnita, L.
    Selective recruitment of G protein-coupled receptor kinases (GRKs) controls signaling of the insulin-like growth factor 1 receptor
    (2012) Proceedings of the National Academy of Sciences of the United States of America, 109 (18), pp. 7055-7060.

    Quintana-Portillo, R., Canfrán-Duque, A., Issad, T., Sánchez-Margalet, V., González-Yanes, C.
    Sam68 interacts with IRS1
    (2012) Biochemical Pharmacology, 83 (1), pp. 78-87.


    Boubekeur S, Boute N, Pagesy P, Zilberfarb V, Christeff N, Issad T.A new highly efficient substrate-trapping mutant of protein tyrosine phosphatase 1B (PTP1B) reveals full autoactivation of the insulin receptor precursor. (2011) J Biol Chem. 286:19373-80.


    Browaeys-Poly E, Blanquart C, Perdereau D, Antoine AF, Goenaga D, Luzy JP, Chen H, Garbay C, Issad T, Cailliau K, Burnol AF. Grb14 inhibits FGF receptor signaling through the regulation of PLCγ recruitment and activation. (2010) FEBS Lett.  584:4383-8.

    Issad T, Masson E, Pagesy P.O-GlcNAc modification, insulin signaling and diabetic complications.(2010) Diabetes Metab.  36:423-35

    Versteyhe, S., Blanquart, C., Hampe, C., Shaukat, M., Christeff, N., de Meyts, P., G. Gray, S.G., and Issad, T. Insulin receptor substrates-5 and -6 are poor substrates for the insulin receptor (2010) Molecular Medicine Reports 3: 189-193, 2010

    Blanquart, C., Karouri, S-E., Issad, T. Protein tyrosine phosphatase-1B and T-Cell protein tyrosine phosphatase regulate IGF2-induced MCF-7 cell migration (2010)  Bioch. Biophys. Res. Comm. 392, 83–88


    Goenaga, D., Hampe, C., Carré, N., Cailliau, K., Browaeys-Poly, E.,  Perdereau, D., Holt, L.J., Daly, R.J., Girard, J., Broutin, I., Issad, T.,  and Burnol, A-F. Molecular determinants of Grb14-mediated inhibition of insulin signaling (2009)  Mol. Endocrinol. 23(7): 1043–1051

     Blanquart, C., Karouri, S-E., Issad, T. Implication of protein tyrosine phosphatase 1B in MCF-7 cell proliferation and resistance to 4-OH tamoxifen (2009)  Bioch. Biophys. Res. Comm. 387 748–753

     Christeff, N., Broussard, C., 1,2,3, Bertherat, J., Hotellier, F., Camoin, L. and Issad, T.  Analysis of main proteins associated with lipid droplets from peri-Adrenal adipose tissue of patients with Cushing’s syndrome (2009) The Open Proteomics Journal 2, 40-48


    Kuo MS, Zilberfarb V, Gangneux N, Christeff N, Issad T. O-glycosylation of FoxO1 increases its transcriptional activity towards the glucose 6-phosphatase gene. FEBS Letters 2008; 582: 829-34.

     Kuo, MS, Zilberfarb, V., Gangneux, N., Christeff, N. & Issad, T. O-GlcNAc modification of FoxO1 increases its transcriptional activity: A role in the glucotoxicity phenomenon? (2008) Biochimie. 90:679-85.

     Kuo, M.-S., Zilberfarb, V., Gangneux, N., Christeff, N. & Issad, T. A new mode of reglulation of FoxO1 by O-GlcNAc glycosylation: involvement in the glucotoxicity phenomenon. (2008) Med Sci (Paris). 24(4):369-71.

     Blanquart, C., Achi, J. and Issad, T. Characterisation of IRA/IRB insulin hybrid receptors using Bioluminescence Resonance energy transfer (2008) Biochem Pharmacol. 76(7):873-83

     Issad, T. and Kuo, MS (2008) O-GlcNAc modification of transcription factors, glucose sensing and glucotoxicity. Trends Endocrinol. Metabol.(2008) 19(10): 380-389.


    Issad, T., Blanquart, C. and Gonzalez-Yanes, C. The use of Bioluminescence Resonance Energy Transfer (BRET) for the study of therapeutic targets: application to tyrosine kinase receptors (2007) Expert. Opin. Ther. Targets 11: 541-556

     Bermudez-Humaran, L.G., Nouaille, S., Zilberfarb, V., Corthier, G., Gruss, A., Langella, P., and Issad, T. Effects of intranasal administration with Lactococcus lactis secreting leptin on food intake, body weight and immune response in mice (2007) Applied and Environmental Microbiology. 73: 5300-5307

     Amselem, S., Carel, J-C, Deroux, N., Issad, T., Maccari, S., Prevot, V. and Suzanne, C (alphabetical order) Croissance et puberté : Évolutions séculaires, facteurs environnementaux et génétiques Expertises collectives INSERM, Editions INSERM, (2007)


    Nouaille S, Blanquart C, Zilberfarb V, Boute N, Perdereau D, Roix J, Burnol AF, Issad T. (2006) Interaction with Grb14 results in site-specific regulation of tyrosine phosphorylation of the insulin receptor. EMBO Reports. 7: 512-518.

    Issad T.  and Jockers R. Bioluminescence Resonance Energy Transfer (BRET) to monitor  protein-protein interactions (2006) Methods in Molecular Biology: Transmembrane signaling protocols (Humana Press) 332:195-209 (Invited methodological chapter)

    Blanquart C, Gonzalez-Yanes C, Issad T. Monitoring the activation state of insulin/insulin-like growth factor-1 hybrid receptors using bioluminescence resonance energy transfer. (2006) Mol Pharmacol. 70:1802-1811

    Nouaille S, Blanquart C, Zilberfarb V, Boute N, Perdereau D, Burnol AF, Issad T. Interaction between the insulin receptor and Grb14: A dynamic study in living cells using BRET. (2006) Biochem Pharmacol. 72:1355-1366


    Lacasa D, Boute N and Issad T. (2005) Interaction of the insulin receptor with the receptor-like protein tyrosine phosphatases PTPalpha and PTPepsilon in living cells. Mol. Pharmacol. 4:1206-1213.

    Issad T, Boute N, Boubekeur S, Lacasa D. (2005) Interaction of PTPB with the insulin receptor precursor during its biosynthesis in the endoplasmic reticulum. Biochimie. 87:111-116.

    Blanquart C, Boute N, Lacasa D and Issad T. (2005) Monitoring the activation state of the Insulin-like Growth Factor-1 Receptor and its interaction with PTP1B by using the BRET methodology. Mol. Pharmacol. 68: 885-894


    Boute N, Zilberfarb V, Camoin L, Bonnafous S, Le Marchand-Brustel Y, Issad T. (2004) The formation of an intrachain disulfide bond in the leptin protein is necessary for efficient leptin secretion. Biochimie. 86:351-56. 

    Issad T., Jockers R. and Marullo S.: Bioluminescence Resonances Energy Transfer (BRET) based studies of receptor dynamics in living cells with Berthold's Mithras. (2004) BP Lettre  Infos 04, 26/02/04


    Boute N., Boubekeur S., Lacasa D. and Issad T. (2003) Dynamics of Interaction between Insulin Receptor and Protein Tyrosine Phosphatase-1B in Living Cells. EMBO reports  4 : 313-319.

    Issad T., Boute N., Boubekeur B., Lacasa D. and Pernet K. Looking for an insulin pill? Use the BRET methodology! (2003) Diabetes and Metabolism  29: 111-117.


    Grosfeld, A.,. Zilberfarb, V., Turban, S., André, J., Guerre-Millo, M., and Issad, T. (2002) Hypoxia increases leptin expression in human PAZ6 adipose cells Diabetologia 45: 527-530

    Lahlou,N., Issad,T., Carel, J-C., Camoin, L., Lebouc,Y., Roger, M., and Girard, J. (2002) Mutations in the human leptin and leptin receptor genes as models of serum leptin receptor regulation Diabetes 51: 1980-1985

    Boute, N., Jockers, R. and Issad, T. (2002) The use of Resonance Energy Transfer in high-throughput screening: BRET versus FRET. Trends in Pharmacological Sciences 23: 351-354

    Issad, T., Boute, N. and Pernet, K (2002) A homogenous assay to monitor the activity of the insulin receptor using Bioluminescence Resonance Energy Transfer. Biochemical Pharmacology 64: 813-817)

    Guerre-Millo, M., Grosfeld, A., and Issad, T. (2002) Stimulatory effect of hypoxia on leptin release in cultured adipose cells. Obesity Research 10: 856

    Issad T., Boute N, Pernet K. (2002) The activity of the insulin receptor assessed by bioluminescence resonance energy transfer. Ann. N. Y. Acad. Sci. 973: 120-123


    Boute, N., Pernet, K., and Issad, T. (2001) The activation state of the insulin receptor monitored using the Bioluminescence Resonance Energy Transfer methodology Molecular Pharmacology, 60:  640-645 (IF=4.612)

    Zilberfarb, V., Siquier, K., Strosberg, A.D., and Issad, T. (2001) Effect of dexamethasone on adipocyte differentiation markers and tumour necrosis factor alpha expression in human PAZ6 cells Diabetologia, 44 : 377-386  (IF=5.337)

    Tavaré, J.M. and Issad, T. (2001) Two-dimensional phosphopeptide mapping of receptor tyrosine kinases Methods in Molecular Biology 124: 67-85 (Humana Press)