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    Dynamic of the mononuclear phagocyte system: insight from NLO imaging



    Alexandre Boissonnas 

    Alexandre Boissonnas

    Centre d'Immunologie et des maladies infectieuses (CIMI), Paris


    Jeudi 12 octobre 2017 à 12h00


     invité par Catherine Lavazec


    Institut Cochin, 22 rue Méchain, 75014 Paris
    Salle de conférence Rosalind Franklin, 2e étage


    The mononuclear phagocyte system is a major mediator of inflammation. Recent advances in the field highlighted that mononuclear phagocytes have not fully revealed their usefulness, in particular concerning the biological importance of monocytes. The implication of monocyte dysregulation in the etiology of inflammatory diseases requires the integration of 1) their intrinsic plasticity leading to the wide heterogeneity of their descendants 2) their numerous physical compartmentalizations and their high circulating rate due to constitutive exchange between lymphoid and non-lymphoid organs 3) their interactions with the highly specialized tissue-resident macrophages.

    We study mononuclear phagocyte physiopathology, based on an innovative approach of multimodal real-time imaging using non-linear optical processes, giving access to new dimensions in tissue architecture and functional studies. Based on specific transgenic mouse strains, we are now able to distinguish monocyte-derived cells and track their respective behavior in their physiological environment in various pathophysiological contexts.

    Based on this technical innovation we aim proposing a spatio-temporal integrative schema of mononuclear phagocyte behavior in their local environment in order to decipher the molecular and cellular mechanisms leading to their dysregulation.

    • Hamon P, Loyher PL, Baudesson de Chanville C, Licata F, Combadière C and Boissonnas A. CX3CR1-dependent endothelial margination modulates Ly6Chigh monocyte systemic deployment upon inflammation in mice. Blood. 2017 Mar 9;129(10):1296-1307
    • Loyher PL, Rochefort J, Baudesson de Chanville C, Hamon P, Lescaille G, Bertolus C, Guillot-Delost M, Krummel M, Lemoine FM, Combadière C and Boissonnas A. CCR2 influences T regulatory cell migration to tumors and serves as a biomarker of cyclophosphamide sensitivity. Cancer Res. 2016 Sep 28.
    • Rodero MP, Poupel L, Loyher PL, Hamon P, Licata F, Pessel C, Hume DA, Combadière C, Boissonnas A. Immune surveillance of the lung by migrating tissue monocytes. Elife. 2015 Jul 13;4:e07847
    • Chousterman B*, Boissonnas A*#, Poupel L, Baudesson de Chanville C, Adam J, Tabibzadeh N,  Licata F, Lukaszewicz AC, Lombès A, Deterre P, Payen D and Combadière C#. Ly6Chigh monocytes protect against kidney damage during sepsis via a CX3CR1-dependent adhesion mechanism. J Am Soc Nephrol. 2015 Jul 9. * co-authors #corresponding authors
    • Hamon P, Rodero MP, Combadière C and Boissonnas A. Tracking mouse bone marrow monocytes in vivo. J Vis Exp. 2015 Feb 27;(96).Rodero MP, Licata F, Poupel L, Hamon P, Khosrotehrani K, Combadiere C, Boissonnas A. In vivo imaging reveals a pioneer wave of monocyte recruitment into mouse skin wounds. PLoS One. 2014 Oct 1;9(10).


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