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    Small non-coding RNAs in host-bacterial interactions

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    Séminaire de l'Institut Cochin

    Lundi 8 avril 2019 - 12h00 - Salle de conférence Rosalind Franklin, 2ème étage

     


    Lionel Navarro

    Institut de Biologie de l'Ecole Normale Supérieure, Paris

     

     invité par Sandrine Bourdoulous

    Institut Cochin, 22 rue Méchain, 75014 Paris

     

    Résumé

    The team of Lionel Navarro is studying the role and regulation of small RNAs in plant innate immunity and the mechanisms by which bacterial effectors interfere with RNA silencing in plants and humans.

    They recently showed that the miRNA pathway is a major component of antibacterial defense in plant and that bacteria suppress multiple steps of the miRNA pathway. They are now analyzing the modes of action of these bacterial silencing suppressors and whether analogous mechanisms of RNA silencing suppression are used by human pathogenic bacteria.

    In addition, they have shown that DNA demethylation is part of a plant immune response and they have identified functionally relevant defense genes that are directly controlled by this epigenetic regulatory mechanism. They now analyze whether bacterial effectors could have evolved strategies to interfere directly with the transcriptional silencing machinery of plants. They are also investigating the contribution of the RNA-directed DNA methylation pathway in transgenerational immune priming and its eventual implication in the evolution of immune-response genes.

     

    Quelques publications

    • Zervudacki J, Yu A, Amesefe D, Wang J, Drouaud J, Navarro L and Deleris A. Transcriptional control and exploitation of an immune-responsive family of retrotransposons in Arabidopsis. Embo Journal (2018) e98482.
    • Thiébeauld O, Charvin M, Singla Rastogi M, Yang F, Pontier D, Pouzet C, Bapaume L, Li G, Deslandes L, Lagrange T, Alfano J.R, and Navarro L. A bacterial GW-effector targets Arabidopsis AGO1 to promote pathogenicity and induces Effector-triggered immunity by disrupting AGO1 homeostasis. BIORXIV (2017) 215590.
    • Deleris A, Halter T, Navarro L. DNA Methylation and Demethylation in Plant Immunity. Annu Rev Phytopathol (2016) 54: 579-603.
    • Halter T & Navarro L. Multilayer and interconnected post-transcriptional and co-transcriptional control of plant NLRs. Curr Opin Plant Biol (2015) 26: 127-134.
    • Boccara M, Sarazin A, Thiébeauld O, Jay F, Voinnet O, Navarro L, Colot V. The Arabidopsis miR472-RDR6 silencing pathway modulates PAMP- and Effector-triggered immunity through the post-transcriptional control of disease resistance genes. PLOS Pathogens (2014) 10: e1003883.
    • Rogato A, Richard H, Sarazin A, Voss B, Cheminant Navarro S, Champeimont R, Navarro L, Carbone A, Hess WR, Falciatore A. The diversity of small non-coding RNAs in the diatom Phaeodactylum tricornutum. BMC Genomics (2014) 15:698.
    • Celton JM, Gaillard S, Bruneau M, Pelletier S, Aubourg S, Martin-Magniette ML, Navarro L, Laurens F, Renou JP. Widespread anti-sense transcription in apple is correlated with siRNA production and indicates a large potential for transcriptional and/or post-transcriptional control. New Phytol (2014) 203: 287-299.
    • Yu A, Lepère G, Jay F, Wang J, Bapaume L, Wang Y, Abraham AL, Penterman J, Fischer RL, Voinnet O, Navarro L. Dynamics and biological relevance of DNA demethylation in Arabidopsis antibacterial defense. PNAS (2013) 110: 2389-2394.
    • Staiger D, Korneli C, Lummer M, Navarro L. Emerging role for RNA-based regulation in plant immunity. New Phytol (2013) 197: 394-404.
    • Jay F, Renou JP, Voinnet O, Navarro L. Biotic stress-associated microRNAs: identification, detection, regulation, and functional analysis. Methods Mol Biol (2010) 592: 183-202.
    • Navarro L., Jay F., Nomura K., He S.Y., Voinnet O. Suppression of the microRNA pathway by bacterial effector proteins. Science (2008) 321: 964-967.
    • Navarro L, Bari R, Achard P, Lison P, Nemri A, Harverd NP, Jones JD. DELLAs control plant immune responses by modulating the balance of jasmonic acid and salicylic acid signaling. Current Biology (2008) 18: 650-655.
    • Navarro L, Bari R, Robert-Seilaniantz A, Nemri A, Jones JDG. Roles of plant hormones in plant resistance and susceptibility to pathogens. Genomics of Disease (2008) 24: 1-10.
    • Navarro L., Dunoyer P., Jay F., Arnold B., Dharmasiri N., Estelle M., Voinnet O., Jones J.D.G. A plant miRNA contributes to antibacterial resistance by repressing auxin signalling. Science (2006) 312: 436-439.
    • Zipfel C, Robatzek S, Navarro L, Oakeley EJ, Jones JD, Felix G, Boller T. Bacterial disease resistance in Arabidopsis through flagellin perception. Nature (2004) 428: 764-767.
    • Navarro L, Zipfel C, Rowland O, Keller I, Robatzek S, Boller T, Jones JD. The transcriptional innate immune response to flg22. Interplay and overlap with Avr gene-dependent defense responses and bacterial pathogenesis. Plant Physiol (2004) 135: 1113-1128.
    • Navarro L, Zipfel C, Rowland O, Robatzek S, Boller T, Jones JDG. International Society for Molecular Plant-Microbe Interactions. miRNA expression profiling of the Arabidopsis flg22-triggered innate immune response and the Avr9/Cf-9 response in tobacco. Biology of Plant-Microbe Interactions (2003) 4: 237-239.

     

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