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    Visualising how chemoattractants control neutrophil behaviour at sites of inflammation

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    Séminaire de l'Institut Cochin

    Jeudi 28 juin 2018 - 12h00 - Salle de conférence Rosalind Franklin, 2ème étage

     

    Milka Sarris
    Milka Sarris

    Department of Physiology, Development and Neuroscience (PDN),
    University of Cambridge, UK

     

     invitée par Hervé Enslen

    Institut Cochin, 22 rue Méchain, 75014 Paris

     

    Résumé

     Cell movement is essential for animal development, wound healing and defence from infection. We are interested in how cell movement is guided to functional destinations. We focus on cells of the immune system (leukocytes), which are remarkably capable of traversing different tissue environments and migrating on demand to sites where their antimicrobial function is needed.

    Our aim is to understand how these highly motile cells search tissues and read guidance signals. What are the molecular cues that guide leukocytes to areas of infection or injury? How do these signals propagate and become presented in tissue environments? And how is this information processed by leukocytes? In other words, how do gradients of stimuli instruct cell polarity, cytoskeletal dynamics and migration behaviour?

    To address these questions, we exploit the zebrafish larva, whose small size and transparency make it ideal for high-resolution in vivo imaging. We record leukocyte dynamics using advanced microscopy techniques and use quantitative and statistical methods to determine how these are modulated by directional signals. We combine this with a variety of genetic, optogenetic or chemical manipulations, to functionally link molecular, cellular and tissue parameters of leukocyte guidance. Through this integrated approach, our goal is to obtain a better understanding of how leukocytes, and eukaryotic cells in general, interpret complex spatial cues.

     

    Quelques publications

    • Sarris M., Olekhnovitch R., Bousso P. Manipulating leukocyte interactions in vivo through optogenetic chemokine release. Blood 127(23) (2016).
    • Sarris M., Sixt M., Navigating in tissue mazes : chemoattractant interpretation in complex environments, Curr. Opin. Cell Biol.. 36, 93-102 (2015)
    • Murayama E, Sarris M, Redd M, Le Guyader D, Vivier C, Horsley W, Trede N, Herbomel P. NACA deficiency reveals the crucial role of somite-derived stromal cells in haematopoietic niche formation. Nat Commun. 6:8375 (2015).
    • Sarris M., Masson J.B, Maurin D., Van der Aa L.M., Boudinot P., Lortat-Jacob H., Herbomel P. Inflammatory chemokines direct and restrict leukocyte migration within live tissues as glycan-bound gradients, Current Biology, 22, 2375-82 (2012).
    • Munoz-Suano A, Kallikourdis M,  Sarris M., & Betz, A.G. Regulatory T cells suppress arthritis during pregnancy. Journal of Autoimmunity, 38, J103-8 (2012).
    • Sarris M. & Betz A.G. Live imaging of dendritic cell-Treg cell interactions. Methods in Molecular Biology, 707, 83-101 (2011).
    • Sarris M. & Betz A.G. Shine a light: imaging the immune system. European Journal of Immunology 39, 1188-1202 (2009).
    • Sarris M., Andersen K.G., Randow F., Mayr, L. & Betz, A. G. Neuropilin-1 expression on regulatory T cells enhances their interactions with dendritic cells during antigen recognition. Immunity 28, 402-413 (2008).

     

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