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    Epigenetic regulation in spermatogenesis; from stem cell to the next generation

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    Séminaire de l'Institut Cochin

    Jeudi 12 décembre 2019 - 12h00 - Salle de conférence Rosalind Franklin, 2ème étage

     


    Yuki Okada

    Institute for Quantitative Biosciences, The University of Tokyo, Japan

     

     invitée par Julie Cocquet

    Institut Cochin, 22 rue Méchain, 75014 Paris

     

    Résumé

     In postnatal stage, male germ cells undergo dynamic chromatin changes during spermatogenesis. My laboratory has been focusing on two specific spermatogenic stages; spermatogonial stem cells and spermatozoa and investigating their unique epigenetic properties (ref. 1-4). In this seminar, I would like to introduce two topics; The first topic is about spermatogonial stem cells and we recently identified Dec2/Behlh41 as a new suppressor for spermatogonial differentiation via transcriptionally repressing Sohlh1 (ref. 5). We also found a new sub-population of undifferentiated spermatogonia, which are characterized by less frequent cell division and higher stem cell activity. RNA-seq and knockout mice analysis are on-going. The second topic is about sperm-retained histones, whose genomic localization was successfully demonstrated by ChIP-seq analyses combined with a newly developed sperm chromatin solubilization method (ref. 6). We are currently investigating the effect of paternal aging on sperm histone localization, and the results imply the intergenerational effect of sperm histones in progeny’s transcriptional regulation. 

     

    Quelques publications

    1. Aoshima et al. (2013) Establishment of Alternative Culture Method for Spermatogonial Stem Cells Using Knockout Serum Replacement. PLoS ONE, 8:e77715.
    2. Hada et al. (2017) “TH2A is phosphorylated at meiotic centromere by Haspin.” Chromosoma. 126(6):769-780.
    3. Hada et al. (2017) “Identification of a variant-specific phosphorylation of TH2A during spermiogenesis.” Sci Rep. 7:46228.
    4. Okada and Yamaguchi (2017) “Epigenetic modifications and reprogramming in paternal pronucleus: sperm, preimplantation embryo, and beyond.” Cell Mol Life Sci. 74(11):1957-1967.
    5. Makino et al. (2019) “Single cell RNA-sequencing identified Dec2 as a suppressive factor for spermatogonial differentiation by inhibiting Sohlh1 expression.” Sci Rep. 9: 6063.
    6. Yamaguchi et al. (2018) “Re-evaluating the localization of sperm-retained histones revealed the modification-dependent accumulation in specific genome regions.” Cell Reports. 23(13):3920-3932.  

     

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